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Cloning and Expression
Enzymes
BstEII-HF
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BstEII-HF
BstEII-HF from New England Biolabs
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Citations:
(6)
Supplier Page
Supplier Page from
New England Biolabs for
BstEII-HF
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BstEII-HF
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R3162S
Quantity
2000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (6)
(1)
Systematic characterization of the local evolutionary space available to human PKR and vaccinia virus K3
bioRxiv
November 25, 2023
Michael J. Chambers, Sophia Scobell, Meru J. Sadhu
purification
. YCp50-RD was digested with restriction enzymes
AgeI-HF
(
NEB
Cat#
R3552S
) and
BstEII-HF
(
NEB
Cat#
R3162S
), followed by gel
purification
. The digested YCp50-RD and pGAL/CYC1-PKR fragments were joined via Gibson
(2)
LRP8‐mediated selenocysteine uptake is a targetable vulnerability in MYCN‐amplified neuroblastoma
EMBO Molecular Medicine
August 7, 2023
Hamed Alborzinia, Zhiyi Chen, Umut Yildiz, Florencio Porto Freitas, Felix C E Vogel, Julianna Patricia Varga, Jasmin Batani, Christoph Bartenhagen, Werner Schmitz, Gabriele Büchel,
et al.
from the puromycin resistance gene. For this, the plasmid was linearized with
BstEII‐HF
(
NEB
, Cat. No.
R3162
) and
MluI‐HF
(
NEB
, Cat. No. R3198), and the synthetic dsDNA fragment (manufactured by Twist Bioscience,
(3)
Protocol for the High-quality Plasmid Isolation from Different Recalcitrant Bacterial Species: Agrobacterium spp., Rhizobium sp., and Bacillus thuringiensis
Bio Protoc
August 5, 2023
Preshobha Kodackattumannil, Shina Sasi, Saranya Krishnan, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
34860-2.5L-R)
NcoI-HF
(
NEB
, catalog number:
R3193M
), storage at -20 °CBstEII-HF (
NEB
, catalog number:
R3162M
), storage at -20 °CHotStar Taq DNA Polymerase PCR kit (
Qiagen
, catalog number:
203205
)
PCR
primers (Macrogen,
(4)
Elucidation of the binding mode of organic polysulfides on the human TRPA1 receptor
Frontiers in Physiology
June 7, 2023
Balázs Nemes, Szabolcs László, Balázs Zoltán Zsidó, Csaba Hetényi, Adam Feher, Ferenc Papp, Zoltan Varga, Éva Szőke, Zoltán Sándor, Erika Pintér
The final
PCR
products and intact pT31-hTRPA1 plasmid were digested by
BstEII-HF
(
New England Biolabs
R3162
) and
BbvCI
(
New England Biolabs
R0601
) enzymes (Figure 2C), then isolated by
agarose gel electrophoresis
,
(5)
3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM)
International journal of molecular sciences
April 15, 2022
Hansmeier NR, Büschlen IS, Behncke RY, Ulferts S, Bisoendial R, Hägerling R.
PCR
products were digested with
PstI-HF
(R3140,
New England Biolabs
, Ipswich, MA, USA) and
BstEII-HF
(
R3162
,
New England Biolabs
, Ipswich, MA, USA) restriction enzymes for 20 min at 37 °C. Simultaneously, empty
(6)
Tighter αC-helix–αL16-helix interactions seem to make p38α less prone to activation by autophosphorylation than Hog1
Bioscience Reports
April 1, 2016
Masha Tesker, Sadiduddin Edbe Selamat, Jonah Beenstock, Ruchama Hayouka, Oded Livnah, David Engelberg
are listed in Table 2. Then, the DNA was cloned to the vector of interest, using the
BstEII
(
NEB
#
R3162S
) and
BlpI
(
NEB
#
R0585S
) restriction enzymes for HOG1 and the
EcoRI
(
NEB
#
R3101S
) restriction enzyme
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BstEII-HF from New England Biolabs
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